Rasa sastra is an ancient science dealing with various drugs of mineral and metallic origin Tuttha (copper sulphate) is a mineral useful in various clinical conditions externally as well as internally. According to Rasasastra, through the process of Shodhana (purification) and Marana (incineration) the harmful effects of Tuttha bhasma (incinerated copper sulphate) is nullified resulting in the formation of a newer compound that is therapeutically more potent. To prepare Tuttha Bhasma by adopting standard manufacturing procedure explained in Ayurvedic texts as well as to study it’s physical and chemical characters using traditional and modern analytical tools. The pharmaceutical processing of Tuttha bhasma was carried out in three Kukkuta puta (incineration) with a peak temperature of 6100C according to Rasa Tarangini reference. Physico chemical analysis, Energy-Dispersive X-ray Fluorescence (EDXRF), X-ray Diffraction (XRD), Particle Size Analysis (PSA) were conducted. The final product shows presence of nano particles which was confirmed by particle analysis. XRD results of Tuttha bhasma revealed the presence of copper sulphide (covellite) with hexagonal lattice and sodium sulphate with orthorhombic structure. All of the preparation stages and changes in the properties were documented and validated, and they may now be used as a valuable tool for standardization and quality assurance of Tuttha bhasma.
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